SYNJ2BP: Human synaptojanin 2 binding protein
PDB Code: 2JIK, 2JIN
SYNJ2BP (synaptojanin 2 binding protein) contains a single PDZ domain. SYNJ2BP is localized to the mitochondrial outer membrane via a C-terminal transmembrane region, with the PDZ domain facing the cytoplasm where it mediates the recruitment of synaptojanin 2A to mitochondria.It has also been shown that SYNJ2BP interacts with and is involved in the intracellular translocation of activin type II receptors.
We crystallised the PDZ domain of SYNJ2BP using a C-terminal extension of a common PDZ binding motif. This allowed the C-terminus of one SYNJ2BP PDZ domain to bind to the binding site of another, contributing important protein-protein interactions for crystal formation. Our structural analysis shows that the domain is capable of binding to a mode 1 PDZ binding motif, in this case -Glu-Ser-Ser-Ile-COO-.

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