Deoxyribonuclease

Deoxyribonuclease

Source:Bovine Pancrease
Storage:Sealed, Dark, at temperature 2-8℃
Description:Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8. DNase I is activated by bivalent metals, and inhibited by chelating agents such EDTA and sodium dodecyl sulfate. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.Deoxyribonuclease has been chromatographically purified to remove trace contaminating proteases. DNase may be used in clinic to thin and liquefy mucus in the lungs.

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