Pullulanase General description

Systematic Name  : pullulan α-1,6-glucanohydrolase 
Unit Definition: 
One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25 °C.
 Physical form: 
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.2 
Reactions :
 Hydrolysis of (16)-α-D-glucosidic linkages in pullulan [a linear polymer of α-(16)-linked maltotriose units] and in amylopectin and glycogen, and the α- and β-limit dextrins of amylopectin and glycogen 
Applications : 
Pullulanase is use in Production of sweeteners and Ethanol from starch,Pullulanase is use in Production of glucose and maltose Syrup.
Pullulanase is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack a-1,6 linkages, while type II pullulanases are also able to hydrolyse a-1,4 linkages. It is also produced by some other bacteria and archaea. Pullanase is used as a detergent in biotechnology.

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